Description

1. Cancer Biology

Ian A. Cree

2. Principles of Cancer Cell Culture

Ian A. Cree

3. Storage of Cell Lines

Katharine A. Parker

4. Characterization and Authentication of Cancer Cell Lines: An Overview

Yvonne A. Reid

5. Online Verification of Human Cell Line Identity by STR DNA Typing

Wilhelm G. Dirks and Hans G. Drexler

6. Cytogenetic Analysis of Cancer Cell Lines

Roderick A. F. MacLeod and Hans G. Drexler

7. Cell Culture Contamination

Glyn N. Stacey

8. Detecting Mycoplasma Contamination in Cell Cultures by Polymerase Chain Reaction

Cord C. Uphoff and Hans G. Drexler

9. Elimination of Mycoplasma from Infected Cell Lines Using Antibiotics

Cord C. Uphoff and Hans G. Drexler

10. Quality Assurance and Good Laboratory Practice

Louise A. Knight and Ian A. Cree

11. Generation of Lung Cancer Cell Line Variants by Drug Selection or Cloning

Laura Breen, Joanne Keenan, and Martin Clynes

12. Isolation and Culture of Colon Cancer Cells and Cell Lines

Sharon Glaysher and Ian A. Cree

13. Isolation and Culture of Melanoma and Naevus Cells and Cell Lines

Julia K. Soo, Alastair D. MacKenzie Ross, and Dorothy C. Bennett

14. Isolation and Culture of Squamous Cell Carcinomalines

Karin J. Purdie, Celine Pourreyron, and Andrew P. South

15. Isolation and Culture of Ovarian Cancer Cells and Cell Lines

Christian M. Kurbacher, Cornelia Korn, Susanne Dexel, Ulrike Schween, Jutta A. Kurbacher, Ralf Reichelt, and Petra N. Arenz

16. Establishment and Culture of Leukemia-Lymphoma Cell Lines

Hans G. Drexler

17. Isolation of Inflammatory Cells from Human Tumors

Marta Polak

18. Isolation of Endothelial Cells from Human Tumors

Elisabeth Naschberger, Vera S. Schellerer, Tilman T. Rau, Roland S. Croner, and Michael St rzl

19. Cellular Chemosensitivity Assays: An Overview

Venil N. Sumantran

20. Cell Sensitivity Assays: The MTT Assay

J. van Meerlo, G.J.L. Kaspers, and J.Cloos

21. Cell Sensitivity Assays: The ATP-based Tumor Chemosensitivity Assay

Sharon Glaysher and Ian A. Cree

22. The Differential Staining Cytotoxicity (DiSC) Assay: A Review

Larry M. Weisenthal

23. Real-time Cytotoxicity Assays

Donald Wlodkowic, Shannon Faley, Zbigniew Darzynkiewicz, and Jonathan M. Cooper

24. FPurification of Annexin V and its use in the Detection of Apoptotic Cells

Katy M. Coxon, James Duggan, M. Francesca Cordeiro, and Stephen E. Moss

25. Measurement of DNA Damage in Individual Cells Using the Single Cell Gel Electrophoresis (Comet) Assay

Janet M. Hartley, Victoria J. Spanswick, and John A. Hartley

26. Molecular Breakpoint Analysis of Chromosome Translocations in Cancer Cell Lines by Long Distance Inverse-PCR

Bj rn Schneider, Hans G. Drexler, and Roderick A.F. MacLeod

27. Cell Migration and Invasion Assays

K.A. Moutasim, M.L. Nystrom, and G.J. Thomas

28. Angiogenesis Assays

V. Poulaki

29. Flow Cytometric DNA Analysis of Human Cancers and Cell Lines

Sarah A. Krueger and George D. Wi.